進一步提高血清促甲狀腺激素（TSH）檢測的敏感性
增強化學發光酶免疫法測定血清促甲狀腺激素方法的建立及初步應用
通過正交試驗，并經方差分析，獲得增強發光酶免疫檢測法的*實驗條件，將其與TSH-酶聯免疫吸附測定（ELISA）試劑盒相結合，建立TSH的增強化學發光酶免疫檢測法
To improve the sensitivity of measurement of TSH in serum.Methods Establishment and application of a new chemiluminescence enzyme immunoassay to determine human thyrotropin in serum LIANG Yan，LIN Hanhua，WANG Muti. Department of Pediatrics, Tongji Hospital,Tongji Medical University
The authors developed a new enhanced chemiluminescence enzyme immunoassay （ECLIA）, which combined luminol-H2O2-HRP system enhanced by para-iodophenol（PIP） with TSH-ELISA test kit. The optimal experimental conditions of the enhanced chemiluminescence were obtained by orthogonal design and analysis of variance
材料和方法
儀器和試劑：Metrohm Herisau E350B-pH計（瑞士）。魯米諾 （Luminol,Serva） 用pH 10.3、0.05 mol/L 碳酸鹽緩沖液配制成5×10-2 mol/L儲備液放置3天后再以pH 8.2、0.1 mol/L 碳酸氫鈉-氫氧化鈉緩沖液稀釋備用。
將化學發光技術與TSH-ELISA試劑盒相結合，建立了TSH的增強化學發光酶免疫檢測法
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